This is a long-term inquiry activity in which students investigate locations they believe harbor cellulose-digesting microbes, collect samples, isolate them on selective media, and screen them for cellulase activity. These novel microbes may be useful for the production of cellulosic ethanol. In the process they learn about plating techniques, serial dilutions, symbiotic relationships and enzyme specificity. Two methods are provided, one focusing on isolation of pure microbial strains, the other focusing on finding symbiotic communities of microbes.

This well-designed experiment compares CO2 impacts on salt water and fresh water. In a short demonstration, students examine how distilled water (i.e., pure water without any dissolved ions or compounds) and seawater are affected differently by increasing carbon dioxide in the air.

Two simple experiments/demonstrations show the role of plants in mitigating the acidification caused when CO2 is dissolved in water.

This short investigation from Carbo Europe explores how temperature affects the solubility of carbon dioxide in water.